|
|
Physiological, structural, and biochemical leaf traits of selected Poaceae species involved in oxidative stress protection and acclimation to different light conditions
Hunt, Lena Macrie ; Albrechtová, Jana (advisor) ; Lux, Alexander (referee) ; Šantrůček, Jiří (referee)
This thesis examines physiological, structural, and biochemical leaf traits in grasses (Poaceae family) and how they contribute to oxidative stress protection. Light is a major factor contributing both to oxidative stress in plants and the induction of protective mechanisms at the leaf level. The agriculturally important species barley (Hordeum vulgare) was used to investigate responses to varying irradiance and atmospheric CO2 levels. Barley was further used as a model organism to study the influence of different spectral qualities on oxidative protective mechanisms, particularly phenolic compound induction. This thesis also examines the protective leaf functional traits of wild grass species (Nardus stricta, Calamagrostis villosa, Molinia caerulea, and Deschampsia cespitosa) in a high-irradiance arctic-alpine tundra grassland. Phenolic compounds are important protective secondary metabolites in plants that protect against oxidative stress from high irradiance. This thesis evaluates phenolic compounds and their contributions to plant stress tolerance and introduces a novel method of quantifying the histochemical detection of phenolics in leaf cross-sections. Key results indicate that light quantity and quality play major influential roles in the accumulation of phenolic compounds. High irradiance...
|
|
|
Effect of water supply on structure and selected physiological parameters of leaf in woody species.
Horešovská, Michaela ; Lhotáková, Zuzana (advisor) ; Votrubová, Olga (referee)
Woody plants must cope with drought as it is one of the main factors of the ongoing climate change. The ability of woody plants to adapt to this stressor determines whether they will survive in constantly changing environmental conditions. The deciduous trees of European temperate forests are not as adapted to drought as Mediterranean trees and shrubs, and therefore the lack of water is a major stress for them. Leaf turned out to be the most flexible plant organ in its response to drought. The aim of this work is to summarize the knowledge about the effect of water deficit on the anatomical structure of leaf and its selected physiological parameters. The first part of this thesis is focused on the drought affecting European tree species and their various defensive strategies against this factor. It mainly describes the modification of leaf's anatomy structure as their defensive reactions observed in dermal, ground and vascular tissue. The second part of this thesis is devoted to the influence of water deficit on the selected leaf's physiological processes, especially on transpiration and photosynthesis, and the key role of stomata closure on both of these physiological processes. Drought causes changes in leaf anatomical structure and its physiological functions. The leaf's developmental stage at...
|
|
|
Microscopy of leaves from different Bergenia species.
Rulfová, Kateřina ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Plants of the genus Bergenia are part of remedies used in Ayurveda medicine. They also play an important part in traditional healing practice in China, India, Mongolia and Russia. Theoretical part of this thesis sums the newest findings and research results concerning three Bergenia species: Bergenia ciliata (Haw.) Sternb., Bergenia crassifolia (L.) Fritsch a Bergenia ornata Stein. The thesis mainly focuses on their current and potential use in medicine and pharmacy. Bergenia extract is traditionally used for dissolving kidney stones, treating respiratory tract illnesses and to stop bleeding. The most important active substances of these plants, their characteristics and main effects are also noted. Bergenia is an important source of arbutin and bergenin. Bergenin has antitussive, antiflogistic and gastroprotective effects. Arbutin is used to treat urinary tract diseases and in cosmetology to lighten the skin. The experimental part of this thesis includes methods of preparation of permanent microscope slides from leaves of chosen Bergenia species. Photographs have been taken from both permanent and native slides. Anatomy of the leaf and leaf epidermis is described including stomatal index. Presence of calcium oxalate crystals in form of druses is also documented. Basic anatomical features were...
|
|
|
Effect of water supply on structure and selected physiological parameters of leaf in woody species.
Horešovská, Michaela ; Lhotáková, Zuzana (advisor) ; Votrubová, Olga (referee)
Woody plants must cope with drought as it is one of the main factors of the ongoing climate change. The ability of woody plants to adapt to this stressor determines whether they will survive in constantly changing environmental conditions. The deciduous trees of European temperate forests are not as adapted to drought as Mediterranean trees and shrubs, and therefore the lack of water is a major stress for them. Leaf turned out to be the most flexible plant organ in its response to drought. The aim of this work is to summarize the knowledge about the effect of water deficit on the anatomical structure of leaf and its selected physiological parameters. The first part of this thesis is focused on the drought affecting European tree species and their various defensive strategies against this factor. It mainly describes the modification of leaf's anatomy structure as their defensive reactions observed in dermal, ground and vascular tissue. The second part of this thesis is devoted to the influence of water deficit on the selected leaf's physiological processes, especially on transpiration and photosynthesis, and the key role of stomata closure on both of these physiological processes. Drought causes changes in leaf anatomical structure and its physiological functions. The leaf's developmental stage at...
|
|
|
Microscopy of leaves from different Bergenia species.
Rulfová, Kateřina ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Plants of the genus Bergenia are part of remedies used in Ayurveda medicine. They also play an important part in traditional healing practice in China, India, Mongolia and Russia. Theoretical part of this thesis sums the newest findings and research results concerning three Bergenia species: Bergenia ciliata (Haw.) Sternb., Bergenia crassifolia (L.) Fritsch a Bergenia ornata Stein. The thesis mainly focuses on their current and potential use in medicine and pharmacy. Bergenia extract is traditionally used for dissolving kidney stones, treating respiratory tract illnesses and to stop bleeding. The most important active substances of these plants, their characteristics and main effects are also noted. Bergenia is an important source of arbutin and bergenin. Bergenin has antitussive, antiflogistic and gastroprotective effects. Arbutin is used to treat urinary tract diseases and in cosmetology to lighten the skin. The experimental part of this thesis includes methods of preparation of permanent microscope slides from leaves of chosen Bergenia species. Photographs have been taken from both permanent and native slides. Anatomy of the leaf and leaf epidermis is described including stomatal index. Presence of calcium oxalate crystals in form of druses is also documented. Basic anatomical features were...
|
|
|
Mesophyll CO2 conductance in consequence with the leaf anatomy and environmental factors.
Malcová, Kateřina ; Lhotáková, Zuzana (advisor) ; Nátr, Lubomír (referee)
The CO2 diffusion pathway from the atmosphere surrounding the leaf to the chloroplast stroma is composed of several parts, where each part poses certain resistance to the CO2 flux. The CO2 diffusion from the sub-stomatal cavities to the stroma is limited due to mesophyll conductance (gm), which includes CO2 movement in gas, liquid and lipid phases. The mesophyll conductance is influenced by factors that are both internal (e.g. leaf anatomy and leaf age) and external (e.g. irradiance, temperature). Internal factors exhibit rather long-term responses of gm, while external factors are involved in short-term changes. For these rapid changes are probably responsible aquaporins. gm significantly influences the rate of net photosynthesis, almost in the same range as a stomatal conductance does. The limitation of photosynthesis given by mesophyll conductance is not fixed and varies among species and plant functional groups. Due to continual increase of CO2 concentration in the atmosphere, variability in gm can lead to enhanced competitive potencial of some plant species.
|
|
|
Carbon dioxide transport through the hypostomatous plant leaf
NEUWIRTHOVÁ, Jitka
Mesophyll conductance is (together with stomatal conductance) a crucial component of diffusion limitations of photosynthesis and it is important to understand the mechanisms of CO2 fluxes through the leaves. Here I tested a new technique for estimation of drawdown in CO2 concentration across hypostomatous leaves based on carbon isotope composition (13C) of leaf cuticle and cuticular waxes isolated from opposite leaf sides.
|
|
| |
guest ::
